PGP9.5和神经肽Y在双峰驼正常睾丸和隐睾的分布比较

doi:10.11843/j.issn.0366-6964.2015.02.018

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (2): 303-308.doi: 10.11843/j.issn.0366-6964.2015.02.018

PGP9.5和神经肽Y在双峰驼正常睾丸和隐睾的分布比较

袁莉刚^1*，闫振龙²，陶金忠³，刘英^1*，陈国娟¹

（1.甘肃农业大学动物医学院，兰州 730070 ；2.甘肃农业大学生命科学技术学院，兰州 730070；3.宁夏大学动物医学院，银川 750021）

收稿日期:2014-06-16 出版日期:2015-02-23 发布日期:2015-02-23
通讯作者: 袁莉刚， E-mail:yuan2918@126.com；刘英，E-mail：Liuy11@gsau.edu.cn
作者简介:袁莉刚(1974-)，女，甘肃陇南人，教授，主要从事动物发育生物学研究，Tel：0931-7631229
基金资助:
国家自然科学研究基金(31160488)；甘肃省自然科学基金项目(145RJZA223)；甘肃省教育厅(0902B-03)

The Distribution of PGP9.5 and NPY in the Normal Testis and Cryptorchidism of Bactrian Camel

YUAN Li-gang^1*，YAN Zhen-long²，TAO Jin-zhong³，LIU Ying^1*，CHEN Guo-juan¹

(1.College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China；2.College of Life Science and Technology，Gansu Agricultural University，Lanzhou 730070，China；3.College of Veterinary Medicine，Ningxia University，Yinchuan 750021，China)

Received:2014-06-16 Online:2015-02-23 Published:2015-02-23

摘要/Abstract

摘要：

探讨PGP9.5和神经肽Y在双峰驼正常睾丸和隐睾的表达及在精子发生中的作用机制。采集性成熟未交配2~3岁成年双峰驼正常睾丸及隐睾，应用免疫组织化学SP法检测PGP9.5和神经肽Y在睾丸的定位，并通过图像分析技术进行定量分析。结果表明：PGP9.5在正常睾丸支持细胞、各级生精细胞和动静脉血管都有高密度阳性反应；神经肽Y在支持细胞呈中等阳性，各级生精细胞和动静脉血管呈弱阳性，隐睾组中PGP9.5和神经肽Y表达位置相似于正常组，但表达量显著降低。PGP9.5和神经肽Y在正常组和隐睾组Leydig细胞表达无差异，均为强阳性。可见PGP9.5及神经肽Y参与了双峰驼正常睾丸和隐睾生精功能的调节，二者通过支持细胞及管周肌样细胞对于隐睾生精微环境的调控能力降低，但隐睾内间质细胞的分泌并未受明显影响。本研究为进一步研究双峰驼睾丸神经递质变化与隐睾症发生关系及调控机制提供了参考。

Abstract:

The aim of the present study was to investigate the expression of PGP9.5 and NPY in the normal testis and cryptorchidism of Bactrian camel.The cryptorchidism and the normal testis of bactrian camel were studied by immunohistochemical SP technique and IPP (Image-Pro Plus) statistics method.The results showed that the PGP9.5 immunoreactive products distributed strongly in the Sertoli cells，sperm cells，arteries and veins of the normal testis，as well as NPY was moderate positive in Sertoli cell，sperm cells and arteries but weak positive in veins，in addition，Leydig cells in adult camels were strongly immunostained with NPY not only in normal testis but also in cryptorchidism.However，immunoreactivity of PGP9.5 and NPY were detected in cryptorchidism testis with significant differences intensities compared with the normal.These findings suggest that PGP9.5 and NPY are detected in the majority of cells in the seminiferous tubules and the blood vessels are involved in spermatogenesis and secretion of testosterone in Bactrian camel testis，they may play important roles in Bactrian camel male fertility and infertility.

中图分类号:

S852.162

袁莉刚，闫振龙，陶金忠，刘英，陈国娟. PGP9.5和神经肽Y在双峰驼正常睾丸和隐睾的分布比较[J]. 畜牧兽医学报, 2015, 46(2): 303-308.

YUAN Li-gang，YAN Zhen-long，TAO Jin-zhong，LIU Ying，CHEN Guo-juan. The Distribution of PGP9.5 and NPY in the Normal Testis and Cryptorchidism of Bactrian Camel[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 303-308.

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PGP9.5和神经肽Y在双峰驼正常睾丸和隐睾的分布比较

The Distribution of PGP9.5 and NPY in the Normal Testis and Cryptorchidism of Bactrian Camel

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